Hind Limb Ischaemia (HLI)
The hind limb ischaemia angiogenesis experimental model is a well-established and popular “gold standard” tool to test and quantify the effect of novel therapies on the formation and development of new blood vessels – the process known as angiogenesis . This is relevant for therapies where this process should be accelerated e.g. wound healing, or where the opposite is required e.g. to slow or inhibit tumour formation. The model uses Laser Doppler or laser speckle imaging to assess blood perfusion in the hind limbs, one of which is ligated surgically. Dedicated software enables the definition of regions of interest for blood flow assessment on the ischaemic versus non-ischaemic limb to establish a “reperfusion ratio” which can be assessed as often as needed and over a number of days on the same subject. This allows the effect of different therapies that may speed or slow angiogenesis to be quantified. Conveniently, the assessment is non-invasive and does not use tracer dyes.
High resolution laser Doppler imager with best spatial resolution of 100 microns
moorLDI2-HIR - High resolution laser Doppler imager with best spatial resolution of 100 microns
BS1 - moorLDI2 Basic Desktop Stand
DS2 - moorLDI2 Advanced Desktop Stand