moorFLPI

#moorFLPI

Full-field, video frame rate blood flow imaging

  • In a nutshell, moorFLPI-2 is the most user-friendly system for studying cerebral blood flow regulation in rodents.

    Chia-Yi (Alex) Kuan, MD, PhD
    Emory University School of Medicine

  • We can't recommend Moor instruments highly enough. The technology is at the cutting edge and the support second to none.

    Paul Sumners, PhD
    London South Bank University

  • I expect to be using Moor Instrument’s technology for many years to come!

    Faisel Khan, PhD
    Ninewells Hospital & Medical School

  • We have found Moor equipment to be extremely dependable and innovative.

    Dean L. Kellogg, Jr., MD, Ph.D
    University of Texas Health Science Center

  • I cannot rate the company or the staff highly enough.

    Jim House, PhD
    University of Portsmouth

  • Moor Instruments have consistently provided excellent help and support for my research.

    Kim Gooding, PhD
    University of Exeter Medical School

  • Laser Doppler Imager is a standard accurate method we now use in our cerebral blood flow and brain perfusion in our laboratory.

    Momoh A. Yakubu, PhD
    Texas Southern University

  • It goes without saying that the company's imaging technology itself is superb!

    Gourav Banerjee
    Leeds Beckett University

This system has now been superseded by the moorFLPI-2, for full details please click here.

The moorFLPI full field blood perfusion imaging system provides video frame rate images of blood flow in the microvasculature – up to 25 images per second.

An optical zoom function enables resolutions exceeding 1M pixels per cm2. The resulting image is mainly of blood flow in the microvessels in the surface layers of the tissue being sampled for example the nutritional flow in skin. In exposed tissue where blood vessels are close to the surface e.g. open surgery, these will also be imaged. Compared to standard laser Doppler imaging the effective sampling depth is small.

Ideally suited to any application where very dynamic changes are occurring – over a few seconds for example – when conventional laser Doppler imaging could not provide data with sufficient time resolution. It is possible to view pulsation in finger tips (see example below) and variations due to deep breath, occlusion/ ischaemia, reactive hyperaemia etc.

The 60 second example, above, recorded at 5 images per second (maximum 25 images per second) shows reduction in flow due to local occlusion with a pressure cuff and subsequent increase in flow when the cuff is deflated.

The above example shows a 3 day old chick embryo within the egg shell. A small section (about 1.5cm diameter) of the egg shell has been cut away so that the heart and surrounding vessels can be imaged. The example has been recorded at 25 frames per second using the standard spatial mode.

A full range of accessories are available for clinical and research environments.

As with all our products quality and reliability is assured with our standard two year manufacturers warranty.

Please click here for an informative article in the Institute of Physics publication, OLE (Optics and Lasers Europe). The article gives some background to the Full Field technique and offers a comprehensive history, from early beginnings to the current developments at Moor Instruments. Comments are featured from the originator of the technique, Dr David Briers.

This system has now been superseded by the moorFLPI-2, for full details please click here.

The following products are AVAILABLE TO BUY ONLINE and work with the moorFLPI


This system has now been superseded by the moorFLPI-2, for full details please click here.

This section lists the more common questions our customers have about the moorFLPI system. If you have a question you would like answered that does not appear below then please email us. We are happy to help!


Q. What is the penetration depth of moorFLPI?
A. The penetration depth will depend on the optical properties of the tissue sampled as in conventional laser Doppler imaging; however, the signal processed which results in the speckle contrast image is biased towards light coming from the more superficial layers of the tissue. The effective penetration depth will be less than a conventional laser Doppler imager so will focus far more on the nutritive layers and will be less influenced by flow from deeper and larger vessels.

Q. What is the largest area you can image with moorFLPI?
A. The image area is dependent on the optical zoom used and the distance from the scan head to the tissue. This allows full flexibility to study areas from 5mm x 7mm up to 15cm x 20cm.

Q. What is the spatial resolution of the moorFLPI?
A. The moorFLPI offers up to 1M pixels per square cm (50um in spatial mode at up to 25Hz and 10um in temporal mode at up to 1Hz).

Q. How can I assess dynamic responses with moorFLPI?
A. The moorFLPI is used in the same way as a conventional laser Doppler imager - changes from a baseline are commonly assessed. The only difference is in the image acquisition rate with moorFLPI providing 25 images per second opening up many new and exciting research opportunities.

Q. Can I image blood vessels with moorFLPI?
A. Yes, if the blood vessels lie in the surface of the tissue being imaged and if the tissue covering the vessels is effectively transparent to the laser light. This makes the technique ideally suited to cerebral imaging and general open surgery.

Q. How often do I need to calibrate the system?
A. The moorFLPI is factory calibrated. The user can check zeroing and calibration as frequently as required; we recommend to check calibration monthly.

Q. Can I analyse the data from moorFLPI?
A. Images can be analysed in much the same way as a conventional laser Doppler images and sequences allowing comparisons of flow within the same image or in the same subject over time. The video capability also allows the user to define up to 16 regions of interest (that can be varied in size, shape and position) and plot flow in real time from those regions - akin to a 16 channel laser Doppler monitor. Frame grabbing allows the user to select images from the video at flexible or pre defined intervals to build a sequence for analysis.

This system has now been superseded by the moorFLPI-2, for full details please click here.

The list below contains a selection of references citing use of moorFLPI. The list below is a small selection. Please contact us for reference lists on your chosen subject.


Ayata, C. et al., 2004.
Laser speckle flowmetry for the study of cerebrovascular physiology in normal and ischemic mouse cortex.
Journal of cerebral blood flow and metabolism: official journal of the International Society of Cerebral Blood Flow and Metabolism, 24(7), pp.744-55.

Ayata, C. et al., 2004.
Pronounced hypoperfusion during spreading depression in mouse cortex.
Journal of cerebral blood flow and metabolism: official journal of the International Society of Cerebral Blood Flow and Metabolism, 24(10), pp.1172-82.

Bezemer, R. et al., 2010.
Real-time assessment of renal cortical microvascular perfusion heterogeneities using near-infrared laser speckle imaging.
Optics express, 18(14), pp.15054-61.

Bezemer, R. et al., 2010.
Validation of near-infrared laser speckle imaging for assessing microvascular ( re ) perfusion.
Microvascular Research, 79(2), pp.139-143.

Briers, J.D., 2001.
Laser Doppler, speckle and related techniques for blood perfusion mapping and imaging.
Physiological measurement, 22(4), pp.R35-66.

Cheng, H. et al., 2004.
Laser speckle imaging of blood flow in microcirculation.
Physics in Medicine and Biology, 49(7), pp.1347-1357.

Dunn, A.K. et al., 2003.
Simultaneous imaging of total cerebral hemoglobin concentration, oxygenation, and blood flow during functional activation.
Optics letters, 28(1), pp.28-30.

Durduran, T. et al., 2004.
Spatiotemporal Quantification of Cerebral Blood Flow During Functional Activation in Rat Somatosensory Cortex Using.
Blood, pp.518-525.

Dusch, M. et al., 2007.
Rapid flare development evoked by current frequency-dependent stimulation analyzed by full-field laser perfusion imaging.
Neuroreport, 18(11), pp.1101-5.

Gorbach, a. M. et al., 2009.
Functional assessment of hand vasculature using infrared and laser speckle imaging.
Proceedings of SPIE, 7169, pp.716919-716919-9.

Hashimoto, T. et al., 2010.
A Novel Embolic Model of Cerebral Infarction and Evaluation of Stachybotrys microspora Triprenyl Phenol-7 (SMTP-7), a Novel Fungal Triprenyl Phenol Metabolite.
Journal of Pharmacological Sciences, 114(1), pp.41-49.

Hecht, N. et al., 2009.
Intraoperative monitoring of cerebral blood flow by laser speckle contrast analysis.
Neurosurgical focus, 27(4), p.E11.

Holstein-Rathlou, N.-H. et al., 2011.
Nephron blood flow dynamics measured by laser speckle contrast imaging. American journal of physiology.
Renal physiology, 300(2), pp.F319-29.

Klijn, E. et al., 2010.
The effect of perfusion pressure on gastric tissue blood flow in an experimental gastric tube model.
Anesthesia and analgesia, 110(2), pp.541-6.

McGuire, P.G. & Howdieshell, T.R., 2010.
The importance of engraftment in flap revascularization: confirmation by laser speckle perfusion imaging.
The Journal of surgical research, 164(1), pp.e201-12.

Wang, Z. et al., 2010.
What is the optimal anesthetic protocol for measurements of cerebral autoregulation in spontaneously breathing mice? Experimental brain research. Experimentelle Hirnforschung.
Expérimentation cérébrale, 207(3-4), pp.249-58.

This system has now been superseded by the moorFLPI-2, for full details please click here.

Moor Instruments are committed to product development. We reserve the right to change the specifications below without notice.


Measurement Principle

Laser speckle contrast analysis.

Laser Safety Classification

Class 1 per IEC 60825-1:2001 – Safe to use without eye protection.

Calibration

Factory Calibrated using temperature controlled motility standard.

Image size

from 5mm x 7mm up to 15cm x 20cm (continuously variable with zoom lens).

Camera/Image Resolution

576 x 768/ 113 x 152 up to 576 x 768.

Image Acquisition Rate

From 25 images per second to 1 image every 12 hours.

Acquisition Modes

Single Point, Single Image, Repeat Image, Video mode (all simultaneously if needed).

Pixel Resolution

lowest resolution 14,500 pixels per cm² up to highest resolution of 1,000,000 pixels per cm².

Software

Based on Moor Instruments moorLDI software – refined over 15 years according to customer demands including advanced image acquisition, processing, editing, functionality and analysis.

Stand Options

Bench stand, Microstand and Clinical Mobile stand. Scan head has standard camera mount for tripod mounting.

PC Connections

1 x USB and 1 x Firewire (IEEE1394) port. Suit Laptop/ Desktop or Panel PC (no special cards required).

Warranty

2 years, parts & labour, enhanced service contracts available.

Weights/ Dimensions

Scan head 22cm x 23cm x 8cm, Scan head 1.85kg.

Power Supply

Universal Voltage, 100V-230V. Note acquisition rate is unaffected by frequency of local electrical supply.

Approvals

The moorFLPI is designed and built to medical standards. CE marking and registered with the FDA (510(K) Number K063586 for human use in the USA).