High Resolution Laser Doppler Imager

#moorLDI2-HIR

High resolution laser Doppler imager with best spatial resolution of 100 microns

  • I cannot rate the company or the staff highly enough.

    Jim House, PhD
    University of Portsmouth

  • I expect to be using Moor Instrument’s technology for many years to come!

    Faisel Khan, PhD
    Ninewells Hospital & Medical School

  • Laser Doppler Imager is a standard accurate method we now use in our cerebral blood flow and brain perfusion in our laboratory.

    Momoh A. Yakubu, PhD
    Texas Southern University

  • It goes without saying that the company's imaging technology itself is superb!

    Gourav Banerjee
    Leeds Beckett University

  • We have found Moor equipment to be extremely dependable and innovative.

    Dean L. Kellogg, Jr., MD, Ph.D
    University of Texas Health Science Center

  • Moor Instruments have consistently provided excellent help and support for my research.

    Kim Gooding, PhD
    University of Exeter Medical School

  • We can't recommend Moor instruments highly enough. The technology is at the cutting edge and the support second to none.

    Paul Sumners, PhD
    London South Bank University

  • In a nutshell, moorFLPI-2 is the most user-friendly system for studying cerebral blood flow regulation in rodents.

    Chia-Yi (Alex) Kuan, MD, PhD
    Emory University School of Medicine

The moorLDI2-HIR is suitable for a wide range of pre-clinical research investigations, more typically where smaller areas are under investigation. The system features unique focused optics to provide 100 micron pixel size and 256 x 256 pixel resolution for high resolution blood flow images. The scan areas range from just 2.5cm x 2.5cm up to 25cm x 25cm with scan times typically less than 5 minutes. Use of a focussed laser provides a deeper measurement depth, optimal for angiogenesis studies such as hind limb ischemia and tumour modelling and pre-clinical cerebral blood flow imaging. Highly refined image measurement and analysis software allows for flexibility in measurement set up and comprehensive analysis functions. The moorLDI2-HIR features a colour photo image of the scanned area and automatic distance measurement, making the positioning and comparison of images easier.

The system is in routine use in numerous laboratories and clinics globally and employs unique, optical design and signal processing in order to generate the highest resolution and clearest images of its class. Laser Doppler imaging (LDI) is often compared to laser speckle imaging and whilst there are some similarities, both techniques offer unique advantages. LDI (and moorLDI2-HIR in particular) generally offers deeper penetration enabling enhanced visualisation of small vessels below the tissue surface, perfect for pre-clinical studies. For certain applications these features are critical.

Other features and benefits include;

  • Non contact measurement – painless for patient, aids infection control, no chemical tracers or dyes needed.
  • Daylight operation – use in most lab, clinic or theatre settings.
  • Flexible scan sizes – from just 2.5cm x 2.5 cm up to 25cm x 25cm.
  • High spatial resolution – to catch the finest detail to 100 micron.
  • Single and Repeat imaging modes – compare flow from region to region within the same scan and scan the same region repeatedly to assess changes over time.
  • Advanced Windows compatible software – to ease setup and scanning. Post Measurement processing functions to make the most of your data.
  • Protocol control – set the imager to control flexible tissue heating, pressure cuff control and transdermal drug delivery routines – reproducible, precise and reliable.
  • Digital Trigger In/ Out – to synchronise with external devices.
  • Digital Signal Processing and high quality optics – providing the highest sensitivity to changes in blood flow and superb reliability.
  • Choice of stands – for benchtop use.

NOTE: If you are interested in clinical research and larger scan areas please consider the moorLDI2-IR large area imager or the moorFLPI-2 laser speckle imager.

The following products are AVAILABLE TO BUY ONLINE and work with the moorLDI2-HIR


This section lists the more common questions our customers have with regards to the moorLDI2-HIR. If you have a question you would like answered that does not appear below then please email us. We are happy to help!


Q. What is the largest area you can scan in one image?
A. The moorLDI2-HIR - this will scan 2.5cm x 2.5cm with 256 x 256 pixels equating to around 100 pixels for each square mm, maximum area possible with this system is 25cm x 25cm.

Q. What if I need higher resolution than 100 micron per pixel?
A. The moorFLPI-2 will give you higher resolution, up to 9 microns per pixel, for further information on this system, please click here.

Q. What consumables are required for the measurement?
A. The calibration fluid (moorLDI-PFS) has a shelf life of 12 months and needs to be replaced after this time, for a quote, please click here.

There are numerous references where our laser Doppler imagers are cited. The list below is a small selection. Please contact us for reference lists on your chosen subject.


Boden, J., Lassance-Soares, R. M., Wang, H., Wei, Y., Spiga, M-G., Adi, J., Layman, H., Yu, H., Vazquez-Padron, R. I., Andreopoulos, F., and Webster, K. A., (2016).
Vascular Regeneration in Ischemic Hindlimb by Adeno-Associated Virus Expressing Conditionally Silenced Vascular Endothelial Growth Factor.
Journal of the American Heart Assoc, 5(6), e001815.

Chalothorn , D., Clayton , J., Zhang , H., Pomp , D., and Faber , J . E., 2007.
Collateral density, remodeling, and VEGF-A expression differ widely between mouse strains.
Physiological genomics, 30(2), pp.179–91.

Chalothorn , D., Zhang , H., Clayton , J., Thomas , S., and Faber , J . E., 2005.
Catecholamines augment collateral vessel growth and angiogenesis in hindlimb ischemia. American journal of physiology.
Heart and circulatory physiology, 289(2), pp.947–959.

Edmunds, MC., Wigmore, S., Kluth, D., (2013).
In situ transverse rectus abdominis myocutaneous flap: a rat model of myocutaneous ischemia reperfusion injury.
J Vis Exp. 2013 Jun 8;(76). www.jove.com/video/50473/in-situ-transverse-rectus-abdominis-myocutaneous-flap-rat-model

Im, H-J., England, C. G., Feng, L., Graves, S. A., Hernandez, R., Nickles, R. J., Liu, Z., Lee, D. S., Cho, S. Y., and Cai, W. (2016).
Accelerated Blood Clearance Phenomenon Reduces the Passive Targeting of PEGylated Nanoparticles in Peripheral Arterial Disease.
ACS Appl. Mater. Interfaces, 8 (28), pp.17955–17963.

Makarevich , P., Tsokolaeva , Z., Shevelev , A., Rybalkin , I., Shevchenko , E., Beloglazova , I., Vlasik , T., Tkachuk , V., and Parfyonova , Y., 2012.
Combined transfer of human VEGF165 and HGF genes renders potent angiogenic effect in ischemic skeletal muscle.
PloS one, 7(6), p.e38776.

Padgett, M. E., McCord, T. J., McClung, J. M., and Kontos, C. D. , (2016).
Methods for Acute and Subacute Murine Hindlimb Ischemia.
J. Vis. Exp. 112, e54166, doi:10.3791/54166.

Ruvinov , E., Leor , J., and Cohen , S., 2010.
The effects of controlled HGF delivery from an affinity-binding alginate biomaterial on angiogenesis and blood perfusion in a hindlimb ischemia model.
Biomaterials, 31(16), pp.4573–82.

Ryan, T. E., Schmidt, C. A., Alleman, R. J., Tsang, A. M., , Green, T. D., Neufer, P. D., Brown, D. A., and McClung, J. M., (2016).
Mitochondrial therapy improves limb perfusion and myopathy following hindlimb ischemia.
J Mol Cell Cardiol., 1(97), pp:191-196.

Shevchenko , E . K., Makarevich , P . I., Tsokolaeva , Z . I., Boldyreva , M., Sysoeva , V . Y., Tkachuk , V., and Parfyonova , Y . V, 2013.
Transplantation of modified human adipose derived stromal cells expressing VEGF165 results in more efficient angiogenic response in ischemic skeletal muscle.
Journal of translational medicine, 11(1), p.138.

Sönmez , T . T., Vinogradov , A., Zor , F., Kweider , N., Lippross , S., Liehn , E . A., Naziroglu , M., Hölzle , F., Wruck , C., Pufe , T., and Tohidnezhad , M., 2013.
The effect of platelet rich plasma on angiogenesis in ischemic flaps in VEGFR2-luc mice.
Biomaterials, 34(11), pp.2674–82.

Suzuki , H., Shibata , R., Kito , T., Ishii , M., Li , P., Yoshikai , T., Nishio , N., Ito , S., Numaguchi , Y., Yamashita , J . K., Murohara , T., and Isobe , K., 2010.
Therapeutic angiogenesis by transplantation of induced pluripotent stem cell-derived Flk-1 positive cells.
BMC cell biology, 11, p.72.

Wu, J., Hadoke, P. W. F., Takov, K., Korczak, A., Denvir, M. A., and Smith, L. B., (2016).
Influence of Androgen Receptor in Vascular Cells on Reperfusion following Hindlimb Ischaemia.
PLOS ONE, May 9. DOI:10.1371/journal.pone.0154987.

Yang, Y., Xiao, L., Chen, N., Li, Y., Deng, X., Wang, L., Sun., H., and Wu, J., (2016).
Platelet-derived factor V promotes angiogenesis in a mouse hind limb ischemia model.
Journal of Vascular Surger, epub ahead of printing, DOI: http://dx.doi.org/10.1016/j.jvs.2016.03.453.

Moor Instruments are committed to product development. We reserve the right to change the specifications below without notice.

The moorLDI2-HIR is a class IIa device under EC directive 93/42/EEC 14 June 1993 Medical Device Directive.


LASER SOURCE

Infra-Red Laser Diode: 785nm nominal, maximum power 2.5mW
Ocular Hazard Distance 20m.
Class 3R per IEC 60825-1:2014. Complies with FDA performance standards for laser products except for deviations pursuant to Laser Notice No. 50, dated June 24, 2007.
Visible Laser Diode (target beam for infrared systems): 660nm nominal, maximum power 0.25mW
All measurements include cumulative measurement uncertainties and expected increases after manufacture.

PROTECTIVE EYEWEAR REQUIREMENTS

The nominal ocular hazard distance is 20 metres.
Operator protection: OD4, 770-850nm.
Patient protection: OD4, 630-670nm and 770-850nm.

ENVIRONMENT CONDITIONS

Temperature: 15°C to 30°C
Humidity: 20% to 80%
Atmospheric pressure: within the range 86.0 kPa to 106.0 kPa (645mmHg to 795mmHg).
Flammable Anaesthetics: the system must not be operated in the presence of flammable anaesthetics.

BANDWIDTH

Scan rate dependent: low frequency cut-off (3db) 20Hz, 100Hz or 250Hz.
Selectable upper cut-off frequency (0.1db) 3KHz, 15KHz or 22.5KHz.

Default Bandwidth in Bold.

RANGE AND SCAN AREA

At 20cm distance, Normal Area = 2.5cm x 2.5cm; Large Area = 5cm x 5cm
At 30cm distance , Normal Area = 3.4cm x 3.4cm; Large Area = 6.8cm x 6.8cm

SCAN SPEED

Scan speed is approximately 4ms/pixel, 10ms/pixel or 50ms/pixel (at maximum resolution). Scan duration is typically 40 seconds for a 12.5cm x 12.5cm image at 64 x 64 pixel resolution, about 6 minutes for a 50cm x 50cm image at 256 x 256 pixel resolution at 4ms/pixel and 100cm distance.

SPATIAL RESOLUTION

High Resolution Instrument

Up to 256 x 256 pixels (actual measurements not by interpolation): 0.1mm/pixel at 20cm for the ’normal’ size scan.

LIGHTING CONDITION

Normal, ambient room lighting.

MEASUREMENTS

FLUX Accuracy: ± 10% relative to Moor Instruments moorLDI2 standard’
Precision: ± 3% of measurement value
Range: 0-5000PU

CONC Accuracy: ± 10%
Precision: ± 5% of measurement value
Range: 0-5000AU

DC Accuracy: ± 10%
Precision: ± 3%
Range: 0-5000AU

CMOS CAMERA

Colour, Auto Focus, 2592 x 1944 pixel resolution, 1296 x 972 (2 x binned) pixel resolution

SOFTWARE

Windows based control, processing and analysis.

ELECTRICAL SAFETY CLASSIFICATION

Type of protection against electric shock – Class I.
Degree of protection against electric shock – Non-patient contact, no applied part.
Degree of protection against ingress of liquid – IPXO (not protected).
Degree of protection against flammable anaesthetics – equipment not suitable for use in the presence of flammable anaesthetics.
Mode of operation – continuous.

GENERAL

Universal voltage switch mode power supply, 100-230V, 50-60Hz, 50VA power consumption
Scan Head: Dimensions W H D mm 426 x 244 x 300: Weight 9kgs.
Operating temperature: 15-30°C.

STORAGE AND TRANSPORTATION CONDITIONS

Temperature: 0-45°C.
Humidity: 0-80% RH.
Atmospheric Pressure: 50.0-106.0 kPa.