Hind Limb Ischemia (HLI)

The hind limb ischemia angiogenesis experimental model is a well-established and popular „gold standard“ tool to test and quantify the effect of novel therapies on the formation and development of new blood vessels – the process known as angiogenesis . This is relevant for therapies where this process should be accelerated e.g. wound healing, or where the opposite is required e.g. to slow or inhibit tumour formation. The Hind Limb Ischemia (HLI) model uses Laser Doppler imaging (moorLDI2-HIR) or laser speckle contrast imaging (moorFLPI-2) to assess blood perfusion in the hind limbs, one of which is ligated surgically. Dedicated software enables the definition of regions of interest for blood flow assessment on the ischemic versus non-ischemic limb to establish a „reperfusion ratio“ which can be assessed as often as needed and over a number of days on the same subject. This allows the effect of different therapies that may speed or slow angiogenesis to be quantified. Conveniently, the assessment is non-invasive and does not use tracer dyes.


The laser speckle contrast imaging technique (moorFLPI-2) offers the highest spatial resolution and can be used to assess reperfusion in the pads of the feet, an alternative technique that avoids the need for limb hair removal. We also offer the high resolution laser Doppler imager (moorLDI2-HIR) with a fine pixel resolution of 100µm and a superior penetration that is ideal for imaging the process and also allows imaging of collateral vessel formation.

Both systems are supplied with unique, advanced software to allow you to visualise, quantify and present your data easily, robustly and quickly.

Hochauflösende Laser-Doppler-Bildgebung

Hochauflösender Laser-Doppler-Imager mit einer räumlichen Auflösung von 50µm

moorLDI2-HIR | Hochauflösender Laser-Doppler-Imager
Laser Speckle Contrast Imager


moorFLPI-2 | Laser Speckle Imager

Was kommt als Nächstes?

Contact us to discuss your specific needs and to request your copy of our free Application Note which includes a detailed experimental method and practical suggestions. We also offer no obligation on-site visits so you can test the equipment in your facility.


Besnier, M., Gasparino, S., Vono, R., Sangalli, E., Facoetti, A., Bollati, V., Cantone, L., Zaccagnini, G., Maimone, B., Fuschi, P., Da Silva, D., Schiavulli, M., Aday, S., Caputo, M., Madeddu, P., Emanueli, C., Martelli, F., & Spinetti, G., (2018).
MicroRNA-210 enhances the therapeutic potential of bone marrow-derived circulating proangiogenic cells in the setting of limb ischemia.
Molecular Therapy, 26(7), 1694-1705.

Chalothorn , D., Clayton , J., Zhang , H., Pomp , D., and Faber , J . E., 2007.
Collateral density, remodeling, and VEGF-A expression differ widely between mouse strains.
Physiological genomics, 30(2), pp.179–91.

Lee, C-Y., Lin, S-J., Wu, T-C., (2020).
miR-548j-5p Regulates Angiogenesis in Peripheral Artery Disease.
Research Square. Cardiac & Cardiovascular Systems.

Ricard, N., Zhang, J., Zhuang, Z.W., and Simons, M., (2020).
Isoform-Specific Roles of ERK1 and ERK2 in Arteriogenesis.
Cells. 9(1): 38.

Xiong, Y., Chang, L-L., Tran, B., Dai, T., Zhong, R., Mao, Y-C., & Zhu, Y-Z., (2019).
ZYZ-803, a novel hydrogen sulfide-nitric oxide conjugated donor, promotes angiogenesis via cross-talk between STAT3 and CaMKII.
Acta Pharmacologica Sinica volume 41, pages 218–22