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Applications

MCA Occlusion (Stroke Model)

Occlusion of the middle cerebral artery is a commonly used technique for the creation of a stroke model. In order to prove that occlusion has taken place, and confirm ischaemia, we recommend the moorVMS-LDF, moorVMS-PC software, VP10M200ST master probe used with P10d single fibre slave probe with corresponding probe holders. For those using a stereotaxic frame we recommend the use of needle probes, the VP3, VP4 or VP5.

Typical published figures demonstrate that a reduction in monitored blood flow of up to 90% can be observed following successful occlusion.

Sample data with kind permission from Dr C. Gibson, Leicester University, Leicester, UK.

Contact us for an Application Note (including a detailed method and Practical Suggestions) or to discuss your specific needs for MCAO.

References

Gibson C. L., Constantin D., Prior M. J. W., Bath P. M. W., Murphy S. P. 2005 Progesterone suppresses the inflammatory response and nitric oxide synthase-2 expression following cerebral ischemia. Experimental Neurology, 193, pp 522– 530.

Fowler J. C., Gervitz L. M., Hamilton M. E., Walker J. A. 2003 Systemic hypoxia and the depression of synaptic transmission in rat hippocampus after carotid artery occlusion. Journal of Physiology, 550 (Pt 3), pp 961-72

Dinapoli V. A., Rosen C. L., Nagamine T., Crocco T. J. 2006 Selective MCA occlusion: A precise embolic stroke model. Neurosci Methods, 154 (1-2), pp 233-8

For additional information on this Application or advice for tailoring Moor Instruments products to your specific application contact us for more information.