Applications

Angiogenesis

The formation of new blood vessels, neovascularisation, involves the processes of angiogenesis and vasculogenesis and is vital for the healing of wounds. Angiogenesis occurs when local mature endothelial cells, derived from existing blood vessels, form new capillaries via migration, proliferation and differentiation and is often studied using laser Doppler imaging (Bauer et al, 2006).

Laser Doppler imaging has been used in studies of angiogenesis in a range of investigations:

Dermal wound healing in punch wounds (Ebrahimian et al, 2009)
Angiogenesis in the ischaemic flap (Thanik et al, 2010. Isenberg et al, 2009. Bauer et al, 2006)

Moor Instruments produces a high resolution laser Doppler imager, the moorLDI2-HR, offering fine pixel resolution of 100µm, ideal for imaging angiogenesis.

We recommend the moorLDI2-HR system for use in imaging of angiogenesis.

References

Bauer S. M., Goldstein L. J., Bauer R. J., Chen H., Putt M., Velazquez O. C. 2006 The bone marrow-derived endothelial progenitor cell response is impaired in delayed wound healing from ischemia. Journal of Vascular Surgery, 43 (1), pp 134-141

Ebrahimian T. G., Pouzoulet F., Squiban C., Buard V., André M., Cousin B., Gourmelon P., Benderitter M., Casteilla L., Tamarat R. 2009 Cell Therapy Based on Adipose Tissue-Derived Stromal Cells Promotes Physiological and Pathological Wound Healing. Arterioscler Thromb Vasc Biol, 29, pp 503-510

Isenberg J. S., Shiva S., Gladwin M. 2009 Thrombospondin-1–CD47 blockade and exogenous nitrite enhance ischemic tissue survival, blood flow and angiogenesis via coupled NO–cGMP pathway activation. Nitric Oxide, 21, pp 52-62

Thanik V. D., Chang C. C., Lerman O. Z., Greives M. R., Le H., Warren S. M., Schneider R. J., Formenti S. C., Saadeh P. B., Levine J. P. 2010 Cutaneous Low-Dose Radiation Increases Tissue Vascularity through Upregulation of Angiogenic and Vasculogenic Pathways Journal of Vascular Research, 47, pp 472–480

For additional information on this Application or advice for tailoring Moor Instruments products to your specific application contact us for more information.